Double-stranded cucumovirus associated RNA 5: experimental analysis of necrogenic and non-necrogenic variants by temperature-gradient gel electrophoresis
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چکیده
منابع مشابه
Characterization of wine yeasts by temperature gradient gel electrophoresis (TGGE).
18S rDNA from 74 wine yeast strains was amplified by PCR using specific primers, and the products analyzed by temperature gradient gel electrophoresis (TGGE). TGGE is a useful method in screening the genotypes of the wine yeasts. Intraspecific differentiation was achieved on the basis of TGGE in some cases, whereas in others identical bands for strains classified as separate species were obtain...
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results the results showed that nl1-gc/ls2 primer set could yield species-specific amplicons, which were well distinguished and allowed better species discrimination than that generated by the its3-gc/its4 primer set, in both dgge and ttge profiles. all five candida species were discriminated by dgge and ttge using the nl1-gc/ls2 primer set. conclusions comparison of dgge and ttge profiles obta...
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982 BioTechniques Vol. 23, No. 6 (1997) chromosome 22q. Proc. Natl. Acad. Sci. USA 93:6297-6301. 7.Levy, J. 1994. Sequencing the yeast genome: an international achievement. Yeast 10:16891706. 8.Liu, Y.G. and R.F. Whittier. 1995. Thermal asymmetric interlaced PCR: automatable amplification and sequencing of insert end fragments from P1 and YAC clones for chromosome walking. Genomics 25:674-681. ...
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Among established techniques for the identification of either known or new mutations, denaturing gradient gel electrophoresis (DGGE) is one of the most effective. However, conventional DGGE is affected by major drawbacks that limit its routine application: the different denaturant gradient ranges and migration times required for different DNA fragments. We developed a modified version of DGGE f...
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association of satellite rna with cucumber mosaic virus (cmv) leads to either attenuation or enhancement of the viral symptoms. for detection of the satellite rnas in iranian isolates of cmv, plant samples showing viral symptoms were collected from different farms in the provinces of east- and west- azerbayjan and ardebil. these samples were inoculated onto the experimental host plants in green...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 1987
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/15.13.5069